Chang-Gong Liu, Ph.D.

Research Associate Professor
Director, Microarray Shared Resource of Comprehensive Cancer Center

Primary Appointment
Department of Molecular Virology, Immunology and Medical Genetics Human Cancer Genetics Program

Contact Information:

Office phone: 614-292-5982

Lab phone: 614-292-2442

Fax number: 614-292-3312

Address: 275 BRT 460 W. 12th Ave, Columbus, OH 43210

E-mail: chang-gong.liu@osumc.edu

Research Interest:
Functional Cancer Genomics

Research Summary:
Microarray is a powerful high throughput technology that can monitor thousands of gene mRNAs/miRNAs by transcriptional profiling in a single experiment. The Microarray Shared Resource (MASR), sponsored by Comprehensive Cancer Center, is providing the platforms of both Affymetrix GeneChip and in-house custom microarray to meet your flexible needs for cancer research and offers genome-wide analysis on DNA and RNA levels include mRNA transcriptional profiling, microRNA/non-coding small RNA transcriptional profiling, DNA single nucleotide polymorphism (SNP) genotyping and functional genomic DNA copy number. The goal of the MASR is to facilitate cancer research by providing a high quality, fast, cost effective and reliable support for the cancer research communities at the Ohio State University and Nationwide.

Selected Publications (Selected):

1. Stoger, R., Kubicka, P., Liu, C.-G., Kafri, T., Razin, A., Cedar, H. and Barlow, D.P. 1993.  Maternal-specific methylation of the imprinted mouse Igf2r locus identifies the expressed locus as carrying the imprinting signal. Cell 73:61-71.
2. Liu, C.-G., Maercker, C., Castanon, M.J., hauptmann, R. and Wiche, G. 1996.  Human Plectin: Origination of the gene, sequence analysis, and chromosomal localization (8q24).  Proc. Natl. Acad. Sci.  USA 93:4278.
3. Sleat, D., Donnelly, R., Lackland, H., Liu, C.-G., Sohr, R.I., Pullarkat, K. and Lobel, P. 1997.  Association of mutations in a lysosomal protein with classic late-infantile neuronal ceroid lipofusinosis.  Science 277:1802-1805.
4. Istvan, S., Sleat, D., Liu, C.-G., Ludwig, T., Arrgiris 1997.  Efstatiadis and phosphate/insulin-like growth factor II receptor are impaired in lysosomal enzymes transport: Comparison of CI-and CD-MPR deficient mice.  Biochem. J. 330:903-908.
5. Liu, C.-G., Sleat, D., Donnelly, R. and Lobel, P. 1998.  Structural organization of human CLN2, the defective gene in the classic late infantile neuronal ceroid lipofuscinoses.  Genomics 50:206-212.
6. Elghanian, R., Xu, Y., McGowen, J., Seithoff, M., Liu, C.-G., Winick, J., Fuller, N., Ramakrishnan, R., Beuhler, A., Johnson, T., Mazumder, A., Bruch, C.K. 2001.  The use and evaluation of 2+2 photoaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix.  Nucleoside & Nucletide Nucleic Acids, 20 (4-7): 1371-5.
7. Zhang, P., Briones, N., Liu, C.-G., Brush, K. 2001.  Acceleration of nucleic acid hybridization on DNA microarrays driven by ph tunable modifications.  Nucleoside & Nucletide Nucleic Acids, 20(4-7): 1251-4.
8. Chen, X., Ding, Y., Liu, C.-G., Mikhail, S., and Yang, C.S. 2002.  Overexpression of glucose-related protein 94 (Grp94) in esophageal adencarcinomas of rat surgical model and humans.  Carcinogenesis, 23 (1):123-30.
9. Nallur G., Marrero R., Luo C.,  R. Krishna M., Bechtel PE, Shao W., Ray M., Wiltshire S., Feng L. Huang H., Liu C.-G., Sun L., Sawyer JR, Kingsmore SF., Schweitzer B. and Xia X., 2003, SNP Genotyping with RCA Signal Amplification on Hydrogel Microarrays. Biomedical Microdevices, 5:2, pp115-123.
10. Fang MZ., Liu C.-G., Song Y., Yang GY, Nie Y., Liao J., Zhao X., Shimada Y., Wang LD, and Yang CS.. Overexpression of Gastrin-Releasing Peptide in Human Esophageal Squamous Cell Carcinomas. Carcinogenesis, 2004 Jun; 25(6):865~871

11.   Liu, C.-G., Calin, G.A., Meloon, B., Gamliel, N., Sevignani, C., Ferracin, M., Dumitru, C.D., Shimizu, M., Zupo, S., Dono, M., Alder, H., Bullrich, F., Negrini, M. and Croce, CM. 2004, An oligonucleotide microchip for genome-wide miRNA profiling in human and mouse tissues. Proc. Natl. Acad. Sci. USA, 101:9740-9744, 2004

12.  Calin, G.A., Liu, C.-G., Sevignani, C., Ferracin, M., Felli, N., Dumitru, C.D., Shimizu, M., Cimmino, A., Zupo, S., Dono, M., Del’Aquila, M.L., Alder, H., Rassenti, L., Kipps, T.J., Bullrich, F., Negrini, M. and Croce,C.M. 2004, MicroRNA profiling reveals distinct signatures in B-cell chronic lymphocytic leukemias.  Proc. Natl. Acad. Sci. USA,. 2004 Aug 10;101(32):11755-60.

13. Calin, G.A., Trapasso, F., Shimizu, M., Dumitru, C.D., Cesari,, R., Yendamuri, S., Godwin, A.K., Ferracin, M., Bernardi, G., Chatterjee, D., Baldassarre, G., Rattan, S., Alder, H., Mabuchi, H., Shiraishi, T., Lotte, L., Vlad Herlea, H., Mauro, F., Dighiero, G., Movsas, B., Rassenti, L., Kipps, T., Baffa, R., Fusco, A., Mori, M., Russo, G., Liu, C.-G., Neuberg, D., Bullrich, F., Negrini, M. and Croce, CM. A germline stop mutation in ARLTS1, a new member of the ADP-ribosylation factor family is associated with familial cancers and reduced in vivo apoptosis. N Engl J Med. 2005 Apr 21;352(16):1667-76
14. Ciafre SA., Galardi S., Mangiola A., Liu C.-G., Sabatino G., Ferracin M., Maira G., Croce CM, Farace MG.. Extensive modulation of a set of microRNAs in primary glioblastoma. 2005, Biochemical and Biophysical Research Communications, 334(2005) 1351-1358
15. Iorio MV., Ferracin M., Liu C.-G., Veronese A., Spizzo R., Sabbioni S., Magri E., Pedriali M., Fabbri M., Campiglio M., Menard S., Rosenberg A., Volinia S., Nenci I., Calin GA., Querzoli P., Negrini M., Croce CM. MicroRNA Gene Expression Deregulation in Human Breast Cancer. Cancer Research, 2005 August, 65 (16) pp7065-7070
16. Liu C.-G., Zhang L., Jiang Y., Chatterjee D., Croce CM, Huebner K. and Fong LYY.. 2005. Modulation of Gene Expression in Precancerous Rat Esophagus by Dietary Zinc Deficit and Replenishment. Cancer Research. 65(17) pp7790-7799.
17. Ghiselli G. and Liu C.-G., 2005, Global Gene Expression Profiling of Cells Overexpressing SMC3. Molecular Cancer 2005, 4:34
18. Duff KT, McAleer MF, Davidson WR., Kari L., Kari C., Liu C.-G., Farber SA, Cheng KC, Mest JR., Wickstrom E., Dicker AP., and Rodeck U.. 2005, Coordinate control of cell cycle regulatory genes in Zebrafish development tested by cyclin D1 knockdown with morpholino phosphorodiamidates and hydroxyprolyl-phosphono peptide nucleic acids. Nucleic Acids Research. 2005 33(15), pp4914-4921.
19. Cimmino A., Calin GA., Fabbri M., Iorio M., Ferracin M., Shimizu M., Wojcik S., Zupo S., Dono M., Rassenti L., Volinia S., Liu C.-G., Kipps TJ., Negrini M., Croce CM. 2005, BCL2 is a target of miR-15 and miR-16, PNAS, 2005 Sept 27, 102(36), pp 13944~13949.
20. Calin GA., Ferracin M., Cimmino A., Leva GD., Shimizu M., Wojcik S., Iorio M., Visione R., Sever NI, Fabbri M., Iuliano R., Palumbo T., Pichiorri F., Roldo C., Garzon R., Sevignani C., Rassenti L., Alder H., Volinia S., Liu C.-G., Kipps TJ, Negrini M., and Croce CM.. 2005, A unique microRNA signature associated with prognostic factors and disease progression in B cell Chronic Lymphocytic Leukemia. N Engl J Med. 353(17), pp21-29
21. Felli N., Fontana L., Pelosi E., Botta R., Bonci D., Facchiano F., Liuzzi F., Lulli V., Morsilli O., Santoro S., Valtieri M.,Calin G. A., Liu C-G., Sorrentino A., Croce C.M., Peschle C.. 2005 MicroRNAs 221 and 222 inhibit normal erythropoiesis and erythroleukemia cell growth via receptor downmodulation. PNAS,  2005 Dec.13, 102(50) pp18081-18086.
22. He H, Jazdzewski K., Li W., Liyanarachchi S., Nagy R., Volinia S., Calin GA., Liu C.-G., Franssila K., Suster S., Kloos RT., Croce CM. and de la Chapelle A.. 2005. The Role of MicroRNA Genes in Papillary Thyroid Cancer, PNAS, 2005 Dec 27;102(52):19075-80. Epub 2005 Dec 19
23. Volinia S., Calin GA., Liu C.G., Ambs S., Cimmino A., Petrocca F., Visione R., Iorio M., Roldo C., Ferracin M, Prueitt RL., Yanaihara N., Lanza G., Scarpa A., Vecchione A., Negrini M., Harris CC, & Croce CM. 2006,  A microRNA expression signature of human solid tumors defines cancer gene targets. PNAS, 2006 February 14, 103(7):2257-2261
24. Shi B, Prisco M, Calin G, Liu C-G, Russo G, Giordano A, Baserga R. 2006. Expression Profiles of microRNA in Proliferating and Differentiating 32D Murine Myeloid Cells. J.Cellular Physiology 2006 February 15
25. Yanaihara N., Caplen N., Bowman E., Kumamoto K, Okamoto A., Yokota J., Tanaka T., Calin GA., Liu C.-G, Croce CM and Harris CC, 2005, microRNA Signature in Lung Cancer Diagnosis and Prognosis, Cancer Cell, March 2006, 9: pp189~198.
26. Garzon R., Pichiorri F., Palumbo T., Luliano R., Cimmino A., Aqueilan R., Volinia S., Bhatt D., Alder H., Marcucci G., Calin GA., Liu C.-G., Bloomfield CD., Andreeff M., and Croce CM. 2006, MicroRNA Fingerprints during human Megakaryocytopoiesis. PNAS, 2006 March 20, 103(13), pp5078~5083.
27. Pallante P.,  Visone R.,  Ferracin M., Ferraro A., Berlingieri MT., Troncone G., Chiappetta G., Liu C.-G, M Santoro^1,2, M Negrini⁴, C M Croce³ and A Fusco^1,2 MicroRNA deregulation in human thyroid papillary carcinomas.  Endocrine-Related Cancer 13 (2) 497-508
28. Roldo C.,  Missiaglia E., Hagan JP., F alconi M., Capelli P., Bersani S., Calin GA, Volinia S., Liu C.-G, Scarpa A., and Croce CM. 2006 MicroRNA Expression Abnormalities in Pancreatic Endocrine and Acinar Tumors are associated with distinctive pathological features and clinical behavior. Journal of Clinical Oncology  Sept. 11, 2006
29. Pekarsky Y., Santanam U., Cimmino A., Palamarchuk A., Efanov A., Maximov V., Volinia S., Alder H., Liu C.-G., Rassenti L., Calin G.A., Hagan J.P., Kipps T.J., and Croce C.M.. Tcl1 Expression in Chronic Lymphocytic Leukemia Is Regulated by miR-29 and miR-181 Cancer Res 2006 66: 11590-11593.
30. Kulshreshtha R, Ferracin M, Wojcik SE, Garzon R, Alder H, Agosto-Perez FJ, Davuluri R, Liu CG, Croce CM, Negrini M, Calin GA, Ivan M. A MicroRNA Signature of Hypoxia. . Mol Cell Biol. 2007 Mar;27(5):1859-67. Epub 2006 Dec 28.

31.   Garzon R, Pichiorri F, Palumbo T, Visentini M, Aqeilan R, Cimmino A, Wang H, Sun H, Volinia S, Alder H, Calin GA, Liu C.-G, Andreeff M and Croce CM. 2007.  MicroRNA gene expression during retinoic acid-induced differentiation of human acute promyelocytic leukemia. Oncogene, January 29, 2007; doi:10.1038/sj.onc.1210186

32. Georgantas RW 3^rd, Hildreth R, Morisot S, Alder J, Liu C-G, Heimfeld S, Calin GA, Croce CM, and Civin CI. CD34+ hematopoietic stem-progenitor cell microRNA expression and function: A circuit diagram of differentiation control. Proc Natl Acad Sci U S A. 2007 Feb 9
33. Bloomston M, Frankel WL, Petrocca F, Volinia S, Alder H, Hagan JP, Liu C-G, Bhatt D, Taccioli C, Croce CM. MicroRNA Expression Patterns to Differentiate Pancreatic Adenocarcinoma from Normal Pancreas and Chronic Pancreatitis. JAMA 2007 May 2;297(17): 1901-8.
34. Blower PE, Verducci JS, Lin S, Zhou J, Chung JH, Dai Z, Liu C.-G, Reinhold W,  Lorenzi PL, Kaldjian EP, Croce CM, Weinstein JN, Sadee W. MicroRNA expression profiles for the NCI-60 cancer cell panel. Molecular Cancer Therapeutics, 2007, May 4^th; [Epub Ahead of  Print]
35. Zuo T., Wang L, Morrison C, Chang X, Zhang H, Li W, Liu Y, Wang Y, Liu X, Chan M W-Y, Liu J.Q, Love R, Liu C.-G, Godfrey V, Shen R, Tim H-M. Huang T H-M, Yang T, Park B.K, Wang C-Y, Zheng P. and Liu Y. FOXP3 is an X-linked breast cancer suppressor gene and an important repressor of the HER-2/ErbB2 oncogene  Cell, in press 2007